|
| Status |
Public on Apr 01, 2007 |
| Title |
K562 Day 4 sample |
| Sample type |
other |
| |
|
| Source name |
K562 cell line (ATCC#CCL-243)
|
| Organism |
Homo sapiens |
| Characteristics |
K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. [Lozzio BB , et al. A multipotential leukemia cell line (K-562) of human origin. Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981. PubMed: 7194480]
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
K562 cells are maintained in IMDM with 10% fetal calf serum (Hyclone).
|
| Growth protocol |
For erythropoietic induction, the cells were adapted to the serum free defined media StemLineII (Sigma) first and then induced to differentiate in the StemLineII media supplemented with 50 ng/ml IL3 and 7.5 U/ml EPO (Mahdi et al. 1998).
Mahdi, T., Alcalay, D., Cognard, C., Tanzer, J., and Kitzis, A. 1998. Rescue of K562 cells from MDM2-modulated p53-dependent apoptosis by growth factor-induced differentiation. Biol. Cell 90: 615-627.
|
| Extracted molecule |
other |
| Extraction protocol |
miRNA extracted from CD34 cells using Ambion miRNA Isolation Kit (Cat#1560)
|
| Label |
Cy3 (Amersham Pharmacia)
|
| Label protocol |
Labeling protocol as described in Ambion miRNA Labeling Kit (Cat#1562)
|
| |
|
| Hybridization protocol |
Hybridization protocols as described in Ambion Probeset (Cat#1561v1) and Ambion miRNA Labeling Kit (Cat#1562)
|
| Scan protocol |
Processed slides were scanned using the microarray scanner GenePix 4000B (Axon), with the lasers power set at maximum with fixed PMT value and a scan resolution of 10 um.
|
| Description |
miRNA expression profiling was performed on ex vivo differentiating erythroid cultures derived from human K562 cells. Day 0 samples were non-stimulated cells. This is used as the baseline for comparison with ex vivo induced erythroid differentiation cultures from Day 2, 4, 6, 8, 10, 15 and 22.
|
| Data processing |
Data in VALUE column are median signal intensity after background subtraction, and normalized by dividing with the average signal (after background subtraction) of the sample
|
| |
|
| Submission date |
Oct 09, 2006 |
| Last update date |
Mar 30, 2007 |
| Contact name |
Meng Ling Choong |
| Organization name |
Experimental Therapeutics Centre
|
| Department |
Drug Discovery
|
| Lab |
Assay Development Group
|
| Street address |
31 Biopolis Way, #03-01 Nanos
|
| City |
Singapore |
| ZIP/Postal code |
138669 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL4400 |
| Series (1) |
| GSE6001 |
miRNA profiling in Human K562 cells undergoing erythropoietic induction |
|
