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Status |
Public on Dec 31, 2018 |
Title |
WM2664-ERK1/2-inhibitor-3hc |
Sample type |
RNA |
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Source name |
WM2664-ERK1/2-inhibitor-3hc
|
Organism |
Homo sapiens |
Characteristics |
cell line: WM266-4 genotype: BRAF V600D treatment: ERK1/2-inhibitor 0.2 uM time point: 3h
|
Treatment protocol |
WM266-4 cells were treated with 0.05uM LGX818, 0.003uM trametinib, 0.3uM MEK162, 0.2uM ERK1/2 inhibitor or DMSO for 3hr or 24hr in triplicates. WM266-4 cells carrying inducible shRNAs were treated with 100ng/ml doxycycline for 3 or 7 days in triplicates.
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Growth protocol |
WM266-4 cells or WM266-6 carrying inducible shRNAs were seeded on 6-well plates at 300,000 cells per well, 24hr before treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with RNAeasy Plus kit (Qiagen) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
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Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
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Description |
ERK1/2 inhibitor treatment 3hr
|
Data processing |
The data were analyzed with Bioconductor affy package (http://bioconductor.org) with RMA method and HGU133Plus2_Hs_ENTREZG custom CDF v16 for target gene definition (Dai et al., 2005)
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Submission date |
May 16, 2014 |
Last update date |
Dec 31, 2018 |
Contact name |
Guoying Karen Yu |
E-mail(s) |
yu.karen@gmail.com
|
Organization name |
Novartis - NIBR
|
Department |
bioinformatics
|
Street address |
4560 Horton St, MS 4.1
|
City |
Emeryville |
State/province |
CA |
ZIP/Postal code |
94608 |
Country |
USA |
|
|
Platform ID |
GPL17810 |
Series (1) |
GSE57721 |
Expression profiling of MAPK inhibition in WM266-4 BRAF V600D mutant melanoma cell line |
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