GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM137150

Query DataSets for GSM137150

Status

Public on Oct 06, 2006

Title

Healthy donor U133Plus-N013

Sample type

RNA

Source name

Bone marrow, healthy donor

Organism

Homo sapiens

Characteristics

Human bone marrow plasma cells, U133Plus-N013

Extracted molecule

total RNA

Label

Biotin

Description

Following Ficoll-Hypaque gradient centrifugation, plasma cells obtained from the bone marrow were isolated from the mononuclear cell fraction by immunomagnetic bead selection using a monoclonal mouse anti-human CD138 antibody (Miltenyi-Biotec, Auburn, CA)
More than 90 percent of the cells used for gene expression profiling were plasma cells, as shown by two-color flow cytometry using CD138+/CD45- and CD38+/CD45- markers, the presence of cytoplasmic immunoglobulin light chains by immunocytochemistry, and morphology by Wright-Giemsa staining

Data processing

GCOS1.1

Submission date

Sep 21, 2006

Last update date

Nov 19, 2015

Contact name

Shaughnessy Jr. John

E-mail(s)

shaughnessyjohn@uams.edu

Phone

(501)296-1503 1410

Organization name

Myeloma Institute for Research and Therapy

Department

Lab

Donna D. and Donald M. Lambert Laboratory of Myeloma Genetics

Street address

4301 West Markham St., Slot 776

City

Little Rock

State/province

ZIP/Postal code

72205

Country

USA

Platform ID

GPL570

Series (1)

GSE5900

Gene Expression of Bone Marrow Plasma Cells from Healthy Donors (N=22), MGUS (N=44), and Smoldering Myeloma (N=12)

Data table header descriptions
ID_REF	probeset name
VALUE	A quantitative measure of the relative abundance of a transcript
ABS_CALL	A qualitative measurement indicating if a given transcript is detected (Present), not detected (Absent), or marginally detected (Marginal)

Data table
ID_REF	VALUE	ABS_CALL
1552256_a_at	647.70001	A
1552257_a_at	1146.1	P
1552258_at	705.29999	P
1552261_at	255.39999	A
1552263_at	244.10001	A
1552264_a_at	394.5	A
1552266_at	81.199997	A
1552269_at	29.299999	A
1552271_at	96.099998	A
1552272_a_at	248.5	A
1552274_at	247.8	A
1552275_s_at	151.8	A
1552276_a_at	41.799999	A
1552277_a_at	1055.7	A
1552278_a_at	393.39999	A
1552279_a_at	1221.9	P
1552280_at	5533.7002	P
1552281_at	230.5	A
1552283_s_at	447.70001	A
1552286_at	219.8	A

Total number of rows: 54675

Table truncated, full table size 1127 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM137150_N013-01-A365-U133Plus-2.cel.gz	5.2 Mb	(ftp)(http)	CEL
Processed data included within Sample table