|
| Status |
Public on Aug 01, 2014 |
| Title |
NB4MTOEcells_ATRA72h |
| Sample type |
RNA |
| |
|
| Source name |
NB4 cells transfected with pcDNA MT-1G expression vector, treated with ATRA for 72 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NB4
genotype/variation: MT-1G-overexpression
|
| Treatment protocol |
NB4 cells transfected with pcDNA empty vector or pcDNA MT-1G expression vector, treated with ATRA for 72 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA Mini Purification Kit (Qiagen, Miami, FL) was used.
|
| Label |
Cy5
|
| Label protocol |
We followed manufacturer's protocol. (http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf)
|
| |
|
| Hybridization protocol |
We followed manufacturer's protocol. (http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf)
|
| Scan protocol |
We followed manufacturer's protocol, and used GenePix 4400A. (http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf)
Raw data files (.TXT) were obtained automatically from CodeLink software. The BLANK and MASK features are absent in this file.
|
| Description |
MTRA
|
| Data processing |
CodeLink Expression Analysis v5.0 software, Quantile normalization
|
| |
|
| Submission date |
Apr 11, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Shinichiro Takahashi |
| Organization name |
Kitasato University
|
| Street address |
1-15-1, kitasato, minami-ku
|
| City |
Sagamihara-City |
| State/province |
Kanagawa-Ken |
| ZIP/Postal code |
252-0373 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE56739 |
A PU.1 suppressive target gene, metallothionein-1G, inhibits retinoic acid-induced NB4 cell differentiation |
|
