Total DNA was isolated according to the manufacturer protocol by using the DNA Blood and Tissue Extraction Kit (Qiagen, Hilde, Germany);
Label
Cy3
Label protocol
not provided
Hybridization protocol
not provided
Scan protocol
Slides were scanned immediately after washing on the Agilent Scanner G2565BA.
Description
array-CGH
Data processing
The scanned images were analyzed with FeatureExtractor (protocol GE1_107_Sep09 and Grid: 014868_D_F_20120131) . Probes were then processed with limma and MANOR using default parameters to remove low-quality probes. Log2 ratios where then median-centered.
