|
| Status |
Public on Sep 01, 2007 |
| Title |
B16-D5-Arm_Day5_95080 |
| Sample type |
RNA |
| |
|
| Source name |
Blood from Rhesus Macaques infected intravenously with a LCMV-Armstrong strain .Sampling is done on the 5th day.
|
| Organism |
Macaca mulatta |
| Characteristics |
healthy adult Rhesus Macaque 5 to 9 years of age infected with LCMV-Armstrong
|
| Treatment protocol |
Rhesus macaques infected intravenously with a dose of LCMV-Armstrong
|
| Growth protocol |
adults infected with LCMV-Armstrong.the LCMV-Arm-infected monkeys did not experience viremia, i.e. viral nucleic acid were detected in tissues but virus loads were not high enough to be detected in the circulation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMC were isolated from blood using density centrifugation. RNA was extracted using Trizol (Gibco-BRL) followed by a Qiagen Rneasy kit to clean the RNA
|
| Label |
biotin
|
| Label protocol |
Staining with R-phycoerythrin-streptavidin (Molecular Probes) was followed by antibody amplification with a biotinylated streptavidin antibody (Vector Laboratories).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning was carried out by using the GeneArray scanner (Hewlett-Packard).
|
| Description |
B16-D5-Arm_Day5_95080
|
| Data processing |
The data were analyzed using the R Bioconductor package using the gcRma algorithm
|
| |
|
| Submission date |
Sep 07, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
kommidi Reddy Chaitanya |
| E-mail(s) |
kommidi@vbi.vt.edu
|
| Phone |
5402311986
|
| Organization name |
VBI
|
| Street address |
Virginia Tech
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24060 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5790 |
Primate blood signs of arenavirus hemorrhagic fever |
|
| Relations |
| Reanalyzed by |
GSE119087 |
