|
| Status |
Public on Mar 18, 2014 |
| Title |
01_CTRL mouse at 48H |
| Sample type |
RNA |
| |
|
| Source name |
Whole bladder 48 hours after sham treatment.
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balbc/A
gender: female
age: Five-week-old
agent: control
tissue: whole bladder
|
| Treatment protocol |
Mice were injected intraperitoneally with 150mg/kg body weight of CYP diluted in saline (CYP group) and mice were injected saline alone as controls (CTRL group). Treated mice were killed 48 hours after administaration and the whole bladders harvested.
|
| Growth protocol |
Mice were housed at a constant room temperature with a cycle of 12-hours light and 12-hours dark. Food and water were available ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Mini kits extraction of total RNA was performed according to the manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ug total RNA (GeneChip® WT Terminal Labeling and Hybridization User Manual, P/N 4425209, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 17 hr at 45C on GeneChip Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affimetrix).
|
| Description |
Gene expression data from the bladder of CTRL mouse
01_CYP0-48H-#21
|
| Data processing |
The data acquisition was performed with the Affymetrix GeneChip Command Console Software and the data were analyzed with GeneSpring GX Version 11.5.1 (Aglient technologies).
Summarization Algorithm ： RMA16, Confidence level ： All, Baseline Options ： Do not perform baseline transformation
|
| |
|
| Submission date |
Mar 18, 2014 |
| Last update date |
Mar 18, 2014 |
| Contact name |
Takeshi Okinami |
| E-mail(s) |
tokinami@kuhp.kyoto-u.ac.jp
|
| Phone |
+81-75-751-3329
|
| Organization name |
Kyoto University Graduate School of Medicine
|
| Department |
Department of Urology
|
| Street address |
54 Shogoin-Kawahara-Cho, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE55986 |
Expression data from mouse whole bladders of cyclophosphamide-induced cystitis |
|
