|
| Status |
Public on Jan 01, 2015 |
| Title |
Mock (buffer) treated chrysanthemum sample_1 |
| Sample type |
RNA |
| |
|
| Source name |
One-month old chrysanthemum plants which were propaged by cutting
|
| Organism |
Chrysanthemum |
| Characteristics |
infection: Mock (buffer) treated
tissue: whole plant
strain: Chrysanthemum (Dendranthema X grandiflora)
|
| Treatment protocol |
Virus infection was performed by sap inoculation.
|
| Growth protocol |
Plants were grown in green house with normal growth condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted by using Qiagen RNeasy Mini Kit.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Samples were harvested at three days after treatment
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Mar 18, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Yeon-Ki Kim |
| E-mail(s) |
kim750a11@gmail.com
|
| Phone |
82-31-321-6351
|
| Organization name |
GreenGene Biotech Inc.
|
| Department |
GreenGene Biotech Inc.
|
| Lab |
Genomics & Genetics Ins.
|
| Street address |
38-2 Namdong
|
| City |
Yongin |
| State/province |
Kyonggido |
| ZIP/Postal code |
449-728 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL18436 |
| Series (1) |
| GSE55985 |
Comparative analysis of chrysanthemum transcriptome in response to infection of three viruses: Cucumber mosaic virus, Tomato spotted wilt virus, and Potato virus X |
|
