|
| Status |
Public on Apr 19, 2014 |
| Title |
BG01_ES-3 |
| Sample type |
RNA |
| |
|
| Source name |
human ES
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BG01 Human embryonic stem cells
|
| Growth protocol |
For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
|
| Label |
biotin
|
| Label protocol |
Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
|
| |
|
| Hybridization protocol |
cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
|
| Scan protocol |
Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
|
| Description |
Gene expression data obtained from human ES
BG_ES_-3
|
| Data processing |
Data processed using AltAnalyze package
|
| |
|
| Submission date |
Mar 07, 2014 |
| Last update date |
Apr 19, 2014 |
| Contact name |
Hu Li |
| E-mail(s) |
li.hu@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Molecular Pharmacology & Experimental Therapeutics
|
| Lab |
Systems Biology and Pharmacology
|
| Street address |
200 First Street, Gonda Building G19-408
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55904 |
| Country |
USA |
| |
|
| Platform ID |
GPL15106 |
| Series (2) |
| GSE55678 |
Alternative Splicing of MBD2 Supports Self-Renewal in Human Pluripotent Stem Cells [HJAY] |
| GSE55679 |
Alternative Splicing of MBD2 Supports Self-Renewal in Human Pluripotent Stem Cells |
|
