|
| Status |
Public on Nov 07, 2014 |
| Title |
parthenogenetic_diploid_ESC_2 |
| Sample type |
RNA |
| |
|
| Source name |
parthenogenetic_diploid_ESC_line2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using Trizol and RNaeasy Purification kit following the manufacturer's recommendations. RNA was quantified using a NanoDrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the One-Color Low RNA Input Amplification kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Cy3-labelled cRNA was fragmented at 60°C for 30 minutes following the manufacturers instructions. After fragmentation reaction, 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Genome Oligo Microarrays for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides.
|
| Description |
gene expression parthenogenetic diploid ESCs
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Feb 27, 2014 |
| Last update date |
Nov 07, 2014 |
| Contact name |
Saori Takahashi |
| E-mail(s) |
saori.takahashi@riken.jp
|
| Phone |
+81-3-5803-4864
|
| Organization name |
Tokyo Medical and Dental University
|
| Department |
Epigenetics
|
| Lab |
Epigenetics
|
| Street address |
1-5-45 Yushima Bunkyo-ku, Tokyo
|
| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (2) |
| GSE55444 |
G2/M transition for stabilizing mouse haploid ESCs: Gene Expression |
| GSE55446 |
G2/M transition for stabilizing mouse haploid ESCs |
|
