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Sample GSM132941 Query DataSets for GSM132941
Status Public on Sep 15, 2006
Title SKI-DLCL cells_Aplidin 3nM_24h_rpt1
Sample type RNA
Source name SKI-DLCL cells
Organism Homo sapiens
Characteristics SKI-DLCL cells treated with Aplidin (3nM) for 24h. Total RNA was extracted.
Biomaterial provider own
Treatment protocol Aplidin alone at concentration 3 nM
Growth protocol Cells were grown under standard cell culture condition (95% air, 5% CO2, 37C)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy kit from Qiagen.
Label Biotin
Label protocol Synthesis of Biotin-Labeled cRNA/Purification

ENZO BioArray RNA Transcript Labeling Kit
GeneChip Sample Cleanup Module

Miscellaneous Reagents/Supplies

100% ethanol room temperature
80% ethanol room temperature
Water, Molecular Biology Grade or DEPC-treated
Ethidium Bromide
Sterile, RNase-free, microcentrifuge tubes, 1.5mL
Sterile-barrier, RNase-free pipette tips
Mini agarose gel electrophoresis unit with appropriate buffers
UV Spectrophotometer
Cooling water bath
Hybridization protocol Hybridization

Water, Molecular Biology Grade or DEPC-treated
Acetylated Bovine Serum Albumin (BSA) solution (50 mg/mL)
Herring Sperm DNA
GeneChip Eukaryotic Hybridization Control Kit
5M NaCl, RNase-free, DNase-free
MES Free Acid Monohydrate
MES Sodium Salt
EDTA Disodium Salt

Miscellaneous Reagents/Supplies

Surfact-Amps 20 (Tween-20), 10%
Hybridization Oven 640
Sterile, RNase-free, microcentrifuge tubes, 1.5mL
Sterile-barrier pipette tips and non-barrier tips
Scan protocol Washing, Staining, and Scanning

Water, Molecular Biology Grade
Distilled water
Acetylated Bovine Serum Albumin (BSA) solution (50 mg/mL)
R-Phycoerythrin Streptavidin
20X SSPE (3M NaCl, 0.2M NaH2PO4, 0.02M EDTA)
Goat IgG, Reagent Grade
Anti-streptavidin antibody (goat), biotinylated
10% surfact-Amps 20 (Tween-20)
Description Samples were processed in CINJ Core Expression Array Facility according to Affymetrix protocol.
Data processing scanned fluorescence intensity, no normalization
Submission date Aug 31, 2006
Last update date Dec 23, 2010
Contact name Pravin Mishra
Organization name UMDNJ
Department Pharmacology
Lab Bertino/Banerjee
Street address 195 Little Albany St.
City New Brunswick
State/province NJ
ZIP/Postal code 08904
Country USA
Platform ID GPL571
Series (1)
GSE5681 Aplidin synergizes with cytosine arabinoside: functional relevance of mitochondria in Aplidin-induced cytotoxicity

Data table header descriptions
VALUE fluorescence intensity values

Data table
AFFX-BioB-5_at 96.3
AFFX-BioB-M_at 122.6
AFFX-BioB-3_at 57.1
AFFX-BioC-5_at 281.1
AFFX-BioC-3_at 309
AFFX-BioDn-5_at 512.9
AFFX-BioDn-3_at 1378.5
AFFX-CreX-5_at 2960.5
AFFX-CreX-3_at 3595.6
AFFX-DapX-5_at 5.3
AFFX-DapX-M_at 4.6
AFFX-DapX-3_at 3.4
AFFX-LysX-5_at 3.1
AFFX-LysX-M_at 0.6
AFFX-LysX-3_at 2.3
AFFX-PheX-5_at 2.8
AFFX-PheX-M_at 0.3
AFFX-PheX-3_at 7.5
AFFX-ThrX-5_at 5.3
AFFX-ThrX-M_at 0.5

Total number of rows: 22277

Table truncated, full table size 342 Kbytes.

Supplementary file Size Download File type/resource
GSM132941.CEL.gz 3.2 Mb (ftp)(http) CEL

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