|
| Status |
Public on Mar 01, 2014 |
| Title |
Mouse heart, IDH2 R140Q mutant, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
IDH2 R140Q mutant mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart
strain: mixed background of C57BL/6, 129/sv, and Balb/c
|
| Treatment protocol |
Mutant mice were given 2mg 4-hydroxytamoxifen (4-OHT) at 5 weeks of age for 3 days
|
| Growth protocol |
Mice were housed in pathogen-free animal facilities at Harvard Medical School and Dana-Farber Cancer Institute
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) followed by RNA cleanup (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix MoGene-1_0-st-v1. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Mouse heart, IDH2 R140Q mutant, replicate 3
|
| Data processing |
Raw expression values were normalized using Robust Multiarray Averaging (RMA) using quantile normalization
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Julian Carretero |
| E-mail(s) |
julian.carretero@uv.es
|
| Organization name |
University of Valencia
|
| Department |
Physiology
|
| Lab |
Molecular Therapies
|
| Street address |
Ave. Vicent Andres Estelles s/n
|
| City |
Burjassot |
| State/province |
Valencia |
| ZIP/Postal code |
46100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE54838 |
D-2-Hydroxyglutarate produced by mutant IDH2 causes cardiomyopathy and neurodegeneration in mice |
|
