|
| Status |
Public on Oct 15, 2006 |
| Title |
WT HSC 3 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow HSC
|
| Organism |
Mus musculus |
| Characteristics |
FACS-sorted Lin-, c-kit+, Sca-1+ HSC from the bone marrow of PU.1 knockdown mice with targeted disruption of the distal enhancer (URE) –14kb upstream of the PU.1 gene.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Micro
|
| Label |
biotin/SAPE
|
| Label protocol |
Amplification of RNA by two rounds of reverse transcription followed by T7 promoter-dependent in-vitro transcription. Then standard labeling according to Affymetrix protocols.
|
| |
|
| Description |
Total RNA from FACS-sorted HSC was amplified by two rounds of RT and T7 promoter-based in-vitro transcription and the resultant biotinylated cRNA was then hybridized to Affymetrix Mouse Genome 430 2.0 arrays.
|
| Data processing |
Array data were analyzed with the dChip software. After smoothing spline normalization, expression values were calculated applying the perfect-match/mismatch difference model algorithm (dChip).
|
| |
|
| Submission date |
Aug 28, 2006 |
| Last update date |
Sep 28, 2006 |
| Contact name |
Ulrich Steidl |
| E-mail(s) |
usteidl@bidmc.harvard.edu
|
| Phone |
617-667-0423
|
| Fax |
617-667-3299
|
| Organization name |
Harvard Institutes of Medicine
|
| Department |
Hematology/Oncology
|
| Lab |
Daniel G. Tenen
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5654 |
Essential role of Jun family transcription factors in PU.1-induced leukemic stem cells |
|
