|
| Status |
Public on Dec 31, 2014 |
| Title |
DNA methylation in RKO |
| Sample type |
genomic |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RKO
|
| Growth protocol |
Du145, PC3, MCF7, MDA-MB-231, and RKO were maintained in RPMI1640 medium. HCT116 was maintained in McCoy's 5A medium. RWPE1 was maintained in keratinocyte-SFM. HMEC was maintained in mammary epithelial cell serum-free growth medium. FHC was maintained in mixture of Ham's F12 and DMEM (1:1).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted by standard phenol/chloroform method, and was quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies).
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Protocol
|
| Scan protocol |
The array was scanned with an iScan System.
|
| Description |
Cancer cell line
|
| Data processing |
The data were analyzed using GenomeStudio Methylation Module Software.
|
| |
|
| Submission date |
Feb 06, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Hideyuki Takeshima |
| E-mail(s) |
takeshima.hideyuki@hoshi.ac.jp
|
| Organization name |
Hoshi University
|
| Department |
Institute for Advanced Life Sciences
|
| Lab |
Department of Epigenomics
|
| Street address |
2-4-41 Ebara
|
| City |
Shinagawa-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
142-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE54758 |
DNA methylation, H3K27me3, and gene expression statuses of human cancer cell lines and normal cells. |
|
