|
| Status |
Public on Jun 12, 2014 |
| Title |
GM02674 |
| Sample type |
RNA |
| |
|
| Source name |
primary fibroblast cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GM02674
cell type: primary fibroblast
blm mutation: Wildtype
clinical features: Not clinically affected
age: 29
gender: Female
|
| Biomaterial provider |
Coriell Cell Repositories http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA02674
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from primary fibroblast cultures when 75 percent confluent. Cells were washed in 1X PBS, scrapped off plates and collected as pellets that were stored at -80 C. Total RNA was isolated using TRIZOL reagent, following the manufacturer’s protocol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix Human Exon 1.0 ST Array through a core facility in the Laboratory of Molecular Technology (NCI-Frederick) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the Laboratory of Molecular Technology (NCI-Frederick).
|
| Description |
primary fibroblast cells
|
| Data processing |
Affymetrix Expression Console Software , RMA normalization, log2
|
| |
|
| Submission date |
Jan 29, 2014 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (2) |
| GSE54502 |
Bloom syndrome protein modulates protein-coding gene and miRNA expression as a function of G4 DNA content |
| GSE62877 |
Werner syndrome WRN helicase alters gene expression in a G-quadruplex DNA-dependent manner to antagonize a pro-senescence gene expression program |
|
