|
Status |
Public on Jan 11, 2014 |
Title |
bipolar_pfc_10 |
Sample type |
RNA |
|
|
Source name |
Pre-frontal cortex (BA46), bipolar disorder
|
Organism |
Homo sapiens |
Characteristics |
age: 52 gender: M race: W pmi: 23.5 ph: 6.7 rin: 7.2 tissue: Pre-frontal cortex (BA46) disease state: bipolar disorder
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Five (5) ug of fragmented, biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133_Plus 2 Gene Chips for 18 hours, then washed/stained according to manufacturer's recommendations using the Affymetrix 450 fluidics station.
|
Scan protocol |
Gene chips were scanned using an Affymetrix 3000 array scanner.
|
Data processing |
R/Bioconductor was used to apply the Robust Multi-Array Average (RMA) methodology to generate expression values. Brain regions were normalized separately.
|
|
|
Submission date |
Jan 10, 2014 |
Last update date |
Jan 11, 2014 |
Contact name |
Thomas A Lanz |
E-mail(s) |
thomas.a.lanz@pfizer.com
|
Phone |
860-441-3551
|
Organization name |
Pfizer
|
Department |
Global Pathology and Investigative Toxicology
|
Street address |
Eastern Point Rd MS# 8274-1224
|
City |
Groton |
State/province |
CT |
ZIP/Postal code |
06340 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE53987 |
Microarray profiling of PFC, HPC and STR from subjects with schizophrenia, bipolar, MDD or control |
|