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Sample GSM1303724 Query DataSets for GSM1303724
Status Public on Mar 04, 2015
Title CK-PAX3-2-12h
Sample type RNA
 
Source name lung from CK-PAX3-infected chicken, 12 hours post infection
Organism Gallus gallus
Characteristics tissue: lung
Extracted molecule total RNA
Extraction protocol Total tissue RNA was extracted using the TRIZOL reagent and purified using QIAGEN RNeasy Mini kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated aRNA were prepared using Genechip IVT Express Kit according to the standard Affymetrix protocol from 1 ug total RNA.
 
Hybridization protocol After fragmentation, 12.5 ug of aRNA were hybridized at 45 ℃ for 16 hr on Affymetrix Genome GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data of lung from CK-PAX3-infected chicken
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jan 09, 2014
Last update date Mar 04, 2015
Contact name Jiao Hu
E-mail(s) hujiao@yzu.edu.cn
Organization name Yangzhou University
Street address 48 Wenhui Road
City Yangzhou
ZIP/Postal code 225009
Country China
 
Platform ID GPL3213
Series (2)
GSE53930 Expression data of lungs from chickens infected with different H5N1 Avian Influenza Viruses [12 hours post infection]
GSE53932 Expression data of lungs from chickens infected with different H5N1 Avian Influenza Viruses

Data table header descriptions
ID_REF
VALUE log2 MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 9.7118435
AFFX-BioB-M_at 10.449456
AFFX-BioB-3_at 9.955689
AFFX-BioC-5_at 11.106641
AFFX-BioC-3_at 11.633005
AFFX-BioDn-5_at 12.760167
AFFX-BioDn-3_at 13.26596
AFFX-CreX-5_at 14.377107
AFFX-CreX-3_at 14.442095
AFFX-DapX-5_at 9.395257
AFFX-DapX-M_at 11.156268
AFFX-DapX-3_at 11.960698
AFFX-LysX-5_at 7.720233
AFFX-LysX-M_at 8.330748
AFFX-LysX-3_at 9.352945
AFFX-PheX-5_at 8.190429
AFFX-PheX-M_at 8.637022
AFFX-PheX-3_at 8.736531
AFFX-ThrX-5_at 6.710298
AFFX-ThrX-M_at 8.459914

Total number of rows: 38535

Table truncated, full table size 1100 Kbytes.




Supplementary file Size Download File type/resource
GSM1303724_CK-PAX3-2.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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