|
| Status |
Public on Mar 04, 2015 |
| Title |
CK10-2-12h |
| Sample type |
RNA |
| |
|
| Source name |
lung from CK10-infected chicken, 12 hours post infection
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total tissue RNA was extracted using the TRIZOL reagent and purified using QIAGEN RNeasy Mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared using Genechip IVT Express Kit according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
After fragmentation, 12.5 ug of aRNA were hybridized at 45 ℃ for 16 hr on Affymetrix Genome GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data of lung from CK10-infected chicken
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 09, 2014 |
| Last update date |
Mar 04, 2015 |
| Contact name |
Jiao Hu |
| E-mail(s) |
hujiao@yzu.edu.cn
|
| Organization name |
Yangzhou University
|
| Street address |
48 Wenhui Road
|
| City |
Yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
|
| Platform ID |
GPL3213 |
| Series (2) |
| GSE53930 |
Expression data of lungs from chickens infected with different H5N1 Avian Influenza Viruses [12 hours post infection] |
| GSE53932 |
Expression data of lungs from chickens infected with different H5N1 Avian Influenza Viruses |
|
