|
| Status |
Public on Aug 23, 2007 |
| Title |
Lymphatic endothelial cells upon conditioned medium from NSCLC hypoxia 24 h |
| Sample type |
RNA |
| |
|
| Source name |
Dermal microvascular cells, commercially available
|
| Organism |
Homo sapiens |
| Characteristics |
RNA Pooled from 6 different individuals
|
| Biomaterial provider |
CAMBREX
|
| Treatment protocol |
Conditioned medium from lung carcinoma cells growth under 1% oxigen, 24 h
|
| Growth protocol |
BEM w/o bFGF and VEGF supplemented with concentrated supernatants from growth media derived from lung adenocarcinoma cells grown under hypoxia for 24 h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol and mini RNeasy kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
IVT labeling kit from affymetrix
|
| |
|
| Hybridization protocol |
Hybridization: According to the manufacturer's protocol (Affymetrix).
|
| Scan protocol |
Scanner: GeneChip Scanner 3000.
|
| Description |
- Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.
|
| Data processing |
Microarray suite 5.0, Tiling Array
|
| |
|
| Submission date |
Aug 23, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ana Rouzaut |
| E-mail(s) |
arouzaut@unav.es
|
| Phone |
34 948194700
|
| Fax |
34 948194714
|
| Organization name |
University of Navarra/CIMA
|
| Department |
Oncology
|
| Lab |
1.03
|
| Street address |
Pio XII 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5579 |
Hypoxia and lymphatic endothelial cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE86362 |
| Reanalyzed by |
GSE119087 |
