Each TaqMan(R) Assay was run in four replicates for each RNA sample using 10 ng total cDNA (measured as total input RNA) in a 10 ul final volume. Assays were run with 2X Universal Master Mix (without UNG) on Applied Biosystems 7900 Fast Real-Time PCR System using universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
Description
NA
Data processing
POLR2A was chosen as the reference gene and each replicate CT was subtracted from the average POLR2A CT to give the log2 difference (delta CT), the normalized value. For delta CT calculations, a CT value of 35 was used for any replicate that had CT > 35.
