Cell lines were grown according to standard conditions (RPMI-1640 glutamax-Gibco/Invitrogen- supplemented with 10% FCS, 100 U/ml penicillin, 100 µg/ml streptomycin, 50 µg/ml gentamycin).
Extracted molecule
total RNA
Extraction protocol
Cells were directly lysed in the culture flasks and total RNA was extracted with RNABee (Campro, Veenendaal, the Netherlands) according to the manufacturer procedure
Label
biotin
Label protocol
Standard Affymetrix protocol
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Data processing
The data were processed using Affymetrix software Expression Console with default analysis settings and RMA as normalization method.
