|
Status |
Public on Nov 01, 2006 |
Title |
H3K36Ac in WT vs genomic DNA replicate 3 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Genomic DNA
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
Genomic DNA
|
Growth protocol |
BY4741 strain cells were grown to OD of 0.8-1, fixed and DNA was purified after reversing crosslinks
|
Extracted molecule |
genomic DNA |
Label |
Cy3
|
Label protocol |
Genomic DNA was labeled using Cy3
|
|
|
Channel 2 |
Source name |
H3K36Ac in WT ChIP DNA
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
ChIP DNA
|
Extracted molecule |
genomic DNA |
Label |
Cy5
|
|
|
|
Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 5.0 analysi
|
Description |
Replicate 3 _array 099
|
Data processing |
After background correction and removal of flagged values, log base 2 expression ratios were median normalized
|
|
|
Submission date |
Aug 15, 2006 |
Last update date |
Aug 23, 2006 |
Contact name |
Jason D Lieb |
E-mail(s) |
jlieb@bio.unc.edu
|
Phone |
919-843-3228
|
Organization name |
UNC-Chapel Hill
|
Street address |
203 Fordham Hall CB# 3280
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599 |
Country |
USA |
|
|
Platform ID |
GPL4075 |
Series (2) |
|