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Status |
Public on Jan 01, 2014 |
Title |
Hs683_rep1_cy3 |
Sample type |
RNA |
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|
Source name |
Hs683_wild type
|
Organism |
Homo sapiens |
Characteristics |
cell line background: Hs683 cell type: glioblastoma cells sample type: TMZ-resistant subclone
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using the miRVana RNA Purification kit (Ambion) according to manufacturer's protocol. RNA was quantified and quality checked with the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
Label |
Cy3
|
Label protocol |
RNA samples were Cy3- or Cy5-labeled using the Agilent Low RNA Input Linear Amplification Kit Plus, according to the manufacturer's protocol.
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|
|
Hybridization protocol |
cRNA was hybridized using the Agilent Gene Expression Hybridization Kit (5188-5242) according to the manufacturer´s protocol.
|
Scan protocol |
Slides were scanned using an Agilent Microarray scanner (G2565BA). Image analysis was performed using feature extraction software version 9.5 (Agilent Technologies). The Agilent GE2-v5_95 protocol was applied using default settings.
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Description |
Hs683_1_cy3 raw data file: US22502676_251485064820_S01_GE2-v5_95_Feb07_1_1.txt
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Data processing |
Array normalization was performed with R-package Limma within the Bioconductor software (v.3.2.3). Two channel raw data has been converted to normalized one channel data.
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Submission date |
Dec 05, 2013 |
Last update date |
Jan 01, 2014 |
Contact name |
Lotte Hiddingh |
E-mail(s) |
l.hiddingh@vumc.nl
|
Organization name |
VU University Medical Center
|
Lab |
Neuro-Oncology Research Group
|
Street address |
De Boelelaan 1117
|
City |
Amsterdam |
ZIP/Postal code |
1081 HV |
Country |
Netherlands |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE53014 |
Gene expression profile of wildtype glioblastoma cell lines and their resistant subclones |
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