|
| Status |
Public on Jan 01, 2014 |
| Title |
U87_rep1_cy3 |
| Sample type |
RNA |
| |
|
| Source name |
U87_wild type
|
| Organism |
Homo sapiens |
| Characteristics |
cell line background: U87
cell type: glioblastoma cells
sample type: TMZ-resistant subclone
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the miRVana RNA Purification kit (Ambion) according to manufacturer's protocol. RNA was quantified and quality checked with the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
RNA samples were Cy3- or Cy5-labeled using the Agilent Low RNA Input Linear Amplification Kit Plus, according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
cRNA was hybridized using the Agilent Gene Expression Hybridization Kit (5188-5242) according to the manufacturer´s protocol.
|
| Scan protocol |
Slides were scanned using an Agilent Microarray scanner (G2565BA). Image analysis was performed using feature extraction software version 9.5 (Agilent Technologies). The Agilent GE2-v5_95 protocol was applied using default settings.
|
| Description |
U87_1_cy3
raw data file:
US22502676_251485064817_S01_GE2-v5_95_Feb07_1_1.txt
|
| Data processing |
Array normalization was performed with R-package Limma within the Bioconductor software (v.3.2.3). Two channel raw data has been converted to normalized one channel data.
|
| |
|
| Submission date |
Dec 05, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Lotte Hiddingh |
| E-mail(s) |
l.hiddingh@vumc.nl
|
| Organization name |
VU University Medical Center
|
| Lab |
Neuro-Oncology Research Group
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE53014 |
Gene expression profile of wildtype glioblastoma cell lines and their resistant subclones |
|
