|
| Status |
Public on Mar 31, 2014 |
| Title |
Human epidermal keratinocyte replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
epidermis
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HEK
agent: none
|
| Treatment protocol |
keratinocytes were cultured for either three or four passes in the presence or absence of 10μM Y27632.
|
| Growth protocol |
Keratinocytes were cultured in F medium [3:1 (v/v) F-12-DMEM, 5% FBS, 0.4μg/ml hydrocortisone, 5μg/ml insulin, 8.4ng/ml cholera toxin, 10ng/ml EGF, 24μg/ml adenine,100U/ml penicillin, 100μg/ml streptomycin] in the presence of irradiated 3T3-J2 feeder cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using RNeasy mini prep kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
cDNA was prepared using the Illumina® TotalPrep™ RNA Amplification Kit (Life Technologies).
|
| |
|
| Hybridization protocol |
gene expression was analyzed by hybridization to Illumina whole genome HumanHT-12 v4.0 BeadChip.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Nov 19, 2013 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Alison A McBride |
| E-mail(s) |
amcbride@niaid.nih.gov
|
| Phone |
3014961370
|
| Organization name |
NIAID NIH
|
| Department |
DIR
|
| Lab |
Lab of Viral Diseases
|
| Street address |
33 North Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE52515 |
Gene regulation in human epidermal keratinocytes by a Rho kinase inhibitor (Y-27632) |
|
