|
| Status |
Public on Oct 25, 2013 |
| Title |
Experimantal rat 5 after 14 months of experiment |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
liver tissue from experimental group
|
| Organism |
Rattus norvegicus |
| Characteristics |
diet: diet enriched with biologicaly active compunds
duration of the experiment: 14 months
tissue: liver
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Animals were divided into two groups: a control group and an experimental group.Rats from both groups were receiving semi-synthetic diets formulated according to the nutritional requirements for laboratory animals and described in. The application of the semi-synthetic diet allowed elimination of the additional impact of biologically active compounds contained in commercially available diets. To experimental diet the following biologically active compounds were added: 6% of salmon fat replacing lard (to increase the level of unsaturated fatty acids), 8% of hydrolysed water extract from small-leaved linden (Tilia cordata) inflorescence (as a source of antioxidant compounds), 8% puree from giant pumpkin (Curcubita macima) (the source of beta-carotene) and 1% of two strains of bacteria with documented probiotic activity: Lactobacillus acidophilus LA-5 and Bifidobacterium animals ssp. lactis. The dry matter content of both diets was the same.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Lipid Tissue Mini Kit following manufacturer's instructions (Qiagen, USA)
|
| Label |
Cy5
|
| Label protocol |
according to the Agilent manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
common reference sample compsed of total RNAs isolated from 10-week healthy rats not participating in the experiment, haused for two weeks in the same room with experimental rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
group: common reference
tissue: liver
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Animals were divided into two groups: a control group and an experimental group.Rats from both groups were receiving semi-synthetic diets formulated according to the nutritional requirements for laboratory animals and described in. The application of the semi-synthetic diet allowed elimination of the additional impact of biologically active compounds contained in commercially available diets. To experimental diet the following biologically active compounds were added: 6% of salmon fat replacing lard (to increase the level of unsaturated fatty acids), 8% of hydrolysed water extract from small-leaved linden (Tilia cordata) inflorescence (as a source of antioxidant compounds), 8% puree from giant pumpkin (Curcubita macima) (the source of beta-carotene) and 1% of two strains of bacteria with documented probiotic activity: Lactobacillus acidophilus LA-5 and Bifidobacterium animals ssp. lactis. The dry matter content of both diets was the same.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Lipid Tissue Mini Kit following manufacturer's instructions (Qiagen, USA)
|
| Label |
Cy3
|
| Label protocol |
according to the Agilent manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
according to the Agilent manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C US10353831 scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Oct 24, 2013 |
| Last update date |
Oct 25, 2013 |
| Contact name |
Hanna Oszkiel |
| Organization name |
WARSAW UNIVERSITY OF LIFE SCIENCES – SGGW (WULS-SGGW)
|
| Department |
Department of Physiological Sciences
|
| Street address |
Nowoursynowska 159
|
| City |
Warsaw |
| ZIP/Postal code |
02 – 776 |
| Country |
Poland |
| |
|
| Platform ID |
GPL15084 |
| Series (1) |
| GSE51657 |
The influence of long-term use of diet enriched with biologically active substances on transcirptomic profile in rats liver |
|
