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Status
Public on Nov 01, 2013
Title
Th17 cells_Donor 6_Adalimumab
Sample type
RNA
Source name
Th17 cells
Organism
Homo sapiens Characteristics
donor: Donor 6
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using the standard TRIzol protocol (Invitrogen, Carlsbad, CA), but with subsequent RNA clean-up of the water phase using the RNeasy Qiagen MinElute kit (Qiagen, Germantown, MD). The quality of the RNA was confirmed on the Agilent Bioanalyzer using RNA Nano chips (Agilent Technologies, Santa Clara, CA).
Label
biotin
Label protocol
Affymetrix in vitro transcription
Hybridization protocol
Affymetrix EukGE-WS2v5
Scan protocol
Affymetrix cel analysis
Data processing
Bioconductor/R RMA
Submission date
Oct 22, 2013
Last update date
Sep 01, 2016
Contact name
Klaus Stensgaard Frederiksen
Organization name
Novo Nordisk A/S
Department
Molecular Genetics
Street address
Novo Nordisk Park F9.1.07
City
Måløv
ZIP/Postal code
DK-2760
Country
Denmark
Platform ID
GPL16268 Series (1)
GSE51540 Effects of TNF-alpha blocking in sorted Th17 cells from co-cultures of human CD4-positive and CD14-positive cells
Relations Reanalyzed by
GSE86362
Data table header descriptions ID_REF VALUE Log2 RMA signal
Data table ID_REF VALUE AFFX-BioB-3_at
4.91344849
AFFX-BioB-5_at
5.234955849
AFFX-BioB-M_at
5.593043751
AFFX-BioC-3_at
9.632960339
AFFX-BioC-5_at
9.225246308
AFFX-BioDn-3_at
11.79539295
AFFX-BioDn-5_at
11.03191256
AFFX-CreX-3_at
13.5694612
AFFX-CreX-5_at
13.20870552
AFFX-DapX-3_at
11.40756162
AFFX-DapX-5_at
7.899047125
AFFX-DapX-M_at
10.2441732
AFFX-HSAC07/X00351_3_at
13.47706952
AFFX-HSAC07/X00351_5_at
11.47931331
AFFX-HSAC07/X00351_M_at
12.86454433
AFFX-HUMGAPDH/M33197_3_at
13.56524621
AFFX-HUMGAPDH/M33197_5_at
13.23862181
AFFX-HUMGAPDH/M33197_M_at
13.40641099
AFFX-HUMISGF3A/M97935_3_at
9.414743833
AFFX-HUMISGF3A/M97935_5_at
5.250390091
19598 591 Kbytes .Supplementary file Size Download File type/resource GSM1247625_1391_HG-U133_Plus_2_.CEL.gz
4.5 Mb
(ftp) (http) CEL
Processed data included within Sample table
