|
| Status |
Public on Sep 26, 2013 |
| Title |
with IL-11 _replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
with IL-11 _replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hESC line H1
|
| Treatment protocol |
bFGF supplement (24ng/ml), IL-11 treatment 10ng/ml.
|
| Growth protocol |
embryonic stem cells grown on matrigel in N2B27 medium supplemented with cytokines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and DNAseI were performed using the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with agarose gel.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 2
SAMPLE 6
|
| Data processing |
The data were normalised using Genome Studio.
|
| |
|
| Submission date |
Sep 25, 2013 |
| Last update date |
Sep 26, 2013 |
| Contact name |
Raed Abu Dawud |
| E-mail(s) |
raed.abu-dawud@charite.de
|
| Organization name |
Berlin-Brandenburg Center for Regenerative Therapies
|
| Department |
Immunology
|
| Lab |
Kurtz
|
| Street address |
Föhrertstr. 15
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE51159 |
Qualitative modeling identifies IL-11 as a novel regulator in maintaining self-renewal in human pluripotent stem cells |
|
