|
| Status |
Public on Jun 04, 2014 |
| Title |
N488T1 |
| Sample type |
RNA |
| |
|
| Source name |
Cord blood_#488_CD4 Tcell_CCRNEg
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: #488
gender: male
tissue: cord blood
cell type: CD4 T cells
cell subset: CCRNEg
|
| Treatment protocol |
Activaiton with antiCD3+antiCD28
|
| Growth protocol |
Culture for 24h in RPMI with 10%FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell samples were lysed using SuperAmp Lysis Buffer following manufacturer’s instructions. SuperAmplification was performed according to Miltenyi Biotec’s undisclosed protocol. Briefly, the amplification is based on a global PCR protocol of mRNA-derived cDNA. mRNA was isolated via magnetic bead technology.
|
| Label |
Cy3
|
| Label protocol |
250 ng of each of the amplified cDNAs were used as template for Cy3 labeling which was performed according to Miltenyi Biotec’s undisclosed protocol.
|
| |
|
| Hybridization protocol |
Cy3 labeled cDNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Human Genome Oligo Microarrays (8x60K) using Agilent’s recommended hybridization chamber and oven. Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
|
| Description |
41
|
| Data processing |
The Agilent Feature Extraction Software (FES 10.7.3.1) was used to read out and process the microarray image files.
|
| |
|
| Submission date |
Sep 24, 2013 |
| Last update date |
Jun 04, 2014 |
| Contact name |
Richard Lo-Man |
| E-mail(s) |
richard.lo-man@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Lab |
Neonatal Immunity group
|
| Street address |
25-28 Rue du Docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL16699 |
| Series (1) |
| GSE51133 |
Analysis of cord blood CD4T cells |
|
