|
| Status |
Public on Nov 03, 2014 |
| Title |
hiPSCs, 59SV9 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
hiPSCs
|
| Organism |
Homo sapiens |
| Characteristics |
source: RP patient (59)
generation method: Sendai virous (iPSCs from fibroblast)
|
| Treatment protocol |
Selected tissues were rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (Invitrogen).
|
| Growth protocol |
Selected tissues were incubated in each medium at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (0.05ug/ul) (3' IVT Express Kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45℃, washed, and stained on GeneChip® Hybridization, Wash and Stain Kit.
|
| Scan protocol |
GeneChips were scanned using the Scanner 3000 7G.
|
| Description |
Gene expression data form hiPSCs (59SV9, Sendai virous)
|
| Data processing |
The data were analyzed with RMA (Rafael A, et al, 2003) algorithm in the R/Bioconductor (http://www.bioconductor.org/) package using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 10, 2013 |
| Last update date |
Nov 03, 2014 |
| Contact name |
Hiroyuki Kamao |
| E-mail(s) |
hiroyuki_retina_galileogalilei@yahoo.co.jp
|
| Organization name |
Kawasaki Medical School
|
| Department |
Ophthalmology
|
| Street address |
577 Matsushima
|
| City |
Kurashiki |
| State/province |
Okayama |
| ZIP/Postal code |
7010192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE50738 |
Expression data from human induced pluripotent stem cells derived retinal pigment epithelium (hiPSC-RPE) |
|
