|
| Status |
Public on Dec 12, 2006 |
| Title |
S4-0 |
| Sample type |
RNA |
| |
|
| Source name |
Time: Day 0; Smoking status: smoker
|
| Organism |
Homo sapiens |
| Characteristics |
Time: Day 0
Smoking status: smoker
Pack-years: 20
Age: 41
Sex: M
Ethnic group: black
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
airway epithelial cells obtained by bronchoscopy and brushing
Time: Day 0; Smoking status: smoker
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) and Robust Multi-array Average (RMA).
|
| |
|
| Submission date |
Jul 21, 2006 |
| Last update date |
Jan 14, 2015 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Genetic Medicine
|
| Lab |
Crystal
|
| Street address |
1300 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5372 |
airway epithelium, large airways, pre and post-mechanical injury |
|
| Relations |
| Reanalyzed by |
GSE64985 |
