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Sample GSM1224951 Query DataSets for GSM1224951
Status Public on Sep 06, 2013
Title Time 30 replicate 3
Sample type RNA
 
Source name Time 30
Organism Homo sapiens
Characteristics cell type: epidermal stem cell
Extracted molecule total RNA
Extraction protocol Total RNAs were prepared with Qiagen Rneasy minikit according to manufacturer’s protocol
Label Cy3
Label protocol 500 ng of total RNA from each sample were amplified by Oligo-dT-T7 reverse transcription and labeled by in vitro transcription with T7 RNA polymerase in the presence of Cy3-CTP using the Quick Amp Labeling kit (Agilent) and purified using RNAeasy columns (Qiagen, Hilden, Germany)
 
Hybridization protocol After fragmentation, 1650 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wahs buffer 2 (Agilent).
Scan protocol Scanned on an Agilent G2539A scanner at 5um resolution and 100%PMT
The intensity data of each individual hybridization were extracted and the quality was assessed with the Feature Extraction software 10.7 (Agilent).
Data processing Raw data was corrected for background noise using the normexp method. Quantile normalization was applied to assure comparability across samples.
 
Submission date Sep 05, 2013
Last update date Sep 06, 2013
Contact name Salvador Aznar Benitah
E-mail(s) salvador.aznar-benitah@irbbarcelona.org
Phone +34 934034021
Organization name Institute for Research in Biomedicine (IRB, Barcelona)
Department Oncology
Lab Stem Cells and Cancer
Street address Baldiri Reixac 10
City Barcelona
ZIP/Postal code 08028
Country Spain
 
Platform ID GPL10332
Series (1)
GSE50631 Human epidermal stem cell function is regulated by circadian oscillations

Data table header descriptions
ID_REF
VALUE normalized log2 intensity values

Data table
ID_REF VALUE
1 18.30597686
2 6.135329656
3 6.109312047
4 6.061865361
5 6.176573534
6 6.111477958
7 6.156900152
8 6.172405515
9 6.179120552
10 6.149459811
11 6.075760208
12 6.227924214
13 9.328618447
14 13.90535222
15 9.776148762
16 14.95237415
17 6.236083734
18 6.482889653
19 13.57579668
20 9.288787656

Total number of rows: 44495

Table truncated, full table size 766 Kbytes.




Supplementary file Size Download File type/resource
GSM1224951_HM_2000252665210327_PHK30h_1_3.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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