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| Status |
Public on Sep 18, 2013 |
| Title |
RNA-Seq (Total RNA) Fetal Stomach; RNA.RS18244 |
| Sample type |
SRA |
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| Source name |
Fetal primary tissue, day 98; RNA.RS18244
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| Organism |
Homo sapiens |
| Characteristics |
sra sample accession: SRS183550
disease: None
biomaterial_provider: University of Washington, Congenital Defects Lab. Ian Glass
biomaterial_type: Primary Tissue
tissue_type: Fetal Stomach
tissue_depot: UW
collection_method: fetal
donor_id: H-23964
donor_age: day 98
donor_health_status: NA
donor_sex: Female
donor_ethnicity: NA
experiment_type: mRNA-Seq
extraction_protocol: Qiagen TissueLyser and RNeasy Lipid Tx Mini kit
extraction_protocol_mrna_enrichment: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
rna_preparation_initial_rna_qnty: 1ug
rna_preparation_reverse_transcription_primer_sequence: Random Primers(hexamers)
rna_preparation_reverse_transcription_protocol: TruSeq RNA
library_generation_pcr_template: cDNA
library_fragmentation: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
library_fragment_size_range: 120-200bp
library_generation_pcr_polymerase_type: Proprietary
library_generation_pcr_thermocycling_program: 98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold
library_generation_pcr_number_cycles: 15
library_generation_pcr_f_primer_sequence: Proprietary
library_generation_pcr_r_primer_sequence: Proprietary
library_generation_pcr_primer_conc: Proprietary
library_generation_pcr_product_isolation_protocol: Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
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| Extracted molecule |
total RNA |
| Extraction protocol |
Library construction protocol: Illumina TruSeq RNA PART# 15008136 REVISION A
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
sample_term_id: UBERON_0000945
assay_term_id: OBI_0001271
nucleic_acid_term_id: SO_0000871
Design description: RNA-Seq (Total RNA) Fetal Stomach
Library name: lib-RNA.RS18244
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.18658
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.7168
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
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| Data processing |
Various levels of processed data files will be made available as this project proceeds.
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| Submission date |
Aug 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
rharris1@bcm.tmc.edu
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| Organization name |
University of Washington
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| Street address |
-
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
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| Relations |
| SRA |
SRX343528 |
| BioSample |
SAMN00254295 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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