|
| Status |
Public on Jul 26, 2013 |
| Title |
U87_BRD4_JQ1_ChipSeq |
| Sample type |
SRA |
| |
|
| Source name |
Glioblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
chip antibody: Brd4
antibody catalog number: Bethyl A301-985A
cell type: U87
duration: 6h
concentration: 500nM
drug: JQ1
|
| Treatment protocol |
500nM JQ1 for 6 hours
|
| Growth protocol |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium (Catalog No. 30-2003)+ 1% GMAX and 10% FBS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Chromatin IP against U87_BRD4_JQ1
|
| Data processing |
Images analysis and base calling was done using the solexa pipeline.
For all samples reads were aligned to their indicated build using bowtie with parameters -e 70 -k 2 -m 2 -n 2 --best --concise. Seed length (-l) was set to read length for each dataset.
Genome_build: hg18 and mm8
Supplementary_files_format_and_content: WIG files: For all samples aligned sequences were extended 150bp upstream and 0bp downstream (with respect to read strand) and allocated into 25bp bins. Counts were normalized to reads per million, and bins with at least 1 read per million are shown.
Genome Build:
01312013_D1TBRACXX_5.CGATGT.wig.gz: hg18
|
| |
|
| Submission date |
Jul 25, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE49224 |
ChIP-Seq for transcriptional regulators and RNA Polymerase II in tumor cells treated with JQ1 |
|
| Relations |
| BioSample |
SAMN02264686 |
| SRA |
SRX327752 |
