|
| Status |
Public on Jun 30, 2009 |
| Title |
Mammary gland, IGF1R-overexpressed, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
OCUBM
|
| Organism |
Homo sapiens |
| Characteristics |
obtained from RIKEN CELL BANK
|
| Treatment protocol |
Cells were treated with Lipofectamine2000 (Invitrogen).
|
| Growth protocol |
DMEM with 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini kit (QIAGEN) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5microg total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
Gene expression data from OCUBM cells transfected with IGF1R.
|
| Data processing |
The data were analyzed with GeneChip Operating Software Ver 1.2.1.001 (GCOS) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Jul 03, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Mayumi Sugimoto |
| E-mail(s) |
m0komats@nlbc.go.jp
|
| Organization name |
National Livstock Breeding Center
|
| Street address |
Odakuraha1
|
| City |
Nishigo |
| ZIP/Postal code |
961-8511 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE5225 |
Expression data from OCUBM cells trasfected with IGF1R |
| GSE5231 |
IGF1R Mediates Mammalian Immune Function in Response to FEZL |
|
| Relations |
| Reanalyzed by |
GSE119087 |
