|
| Status |
Public on Jul 01, 2016 |
| Title |
003793_003793_72deltaMeq(24) |
| Sample type |
RNA |
| |
|
| Source name |
003793_003793_72deltaMeq(24)
|
| Organism |
Gallus gallus |
| Characteristics |
cell type: chicken embryo fibroblast cell line
infection: Meq-null MDV
time (h): 24
|
| Growth protocol |
Cells were cultured in Lebowitz's L-15 and McCoy 5A media with 15% inactivated fetal bovine serum, 100 U of penicillin per ml, and maintained at 37 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Absolutely RNA Miniprep Kit (Stratagene, Santa Clara, CA)
|
| Label |
biotin
|
| Label protocol |
High quality RNA was labeled using Affymetrix (Santa Clara, CA) One-Cycle Target Labeling and Control Reagents
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized to the Affymetrix chips (GeneChip Chicken Genome Array). GeneChips were then washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
Genechips were scanned and the resultant intensities analyzed for levels of transcripts of interest
|
| Data processing |
The data were analyzed with Affymetrix using default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jul 01, 2013 |
| Last update date |
Jul 01, 2016 |
| Contact name |
sugalesini Subramaniam |
| E-mail(s) |
subram28@msu.edu
|
| Phone |
5172302659
|
| Organization name |
Michigan State University
|
| Department |
CMIB
|
| Lab |
USDA-ADOL
|
| Street address |
3606 E Mount Hope Rd
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48823 |
| Country |
USA |
| |
|
| Platform ID |
GPL3213 |
| Series (2) |
| GSE48453 |
Transcriptional Profiling of Meq-dependent Genes in Marek’s Disease Resistant and Susceptible Inbred Chicken Lines |
| GSE48454 |
Genes and pathways involved in cellular transformation by Marek's disease oncoprotein Meq |
|
