|
| Status |
Public on Jun 16, 2006 |
| Title |
Patient ID# M30 |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal muscle taken from rectus abdominis
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
Age(yrs.): 48
|
| Treatment protocol |
Muscle samples were surgically removed from patients, snap-frozen immediately after extraction in liquid nitrogen, and stored at -80C until total RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 8 microg total RNA (Protocol at http://cmgm.stanford.edu/~kimlab/aging_muscle).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix HU-133 2.0 GeneChip array.
|
| Description |
Gene expression data from human skeletal muscle samples.
|
| Data processing |
The data were analyzed and normalized using Dchip. The expression model was positive probe only (PM only). No lower cutoff was used.
|
| |
|
| Submission date |
Jun 15, 2006 |
| Last update date |
Jun 15, 2006 |
| Contact name |
Jacob M Zahn |
| E-mail(s) |
jzahn@stanford.edu
|
| Phone |
650-468-5252
|
| Organization name |
Stanford University
|
| Department |
Developmental Biology
|
| Lab |
Stuart Kim
|
| Street address |
279 Campus Dr.
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5086 |
Transcriptional profile of aging human muscle |
|
