|
| Status |
Public on May 23, 2013 |
| Title |
non tumoral sample 78 |
| Sample type |
RNA |
| |
|
| Source name |
sample of human liver from resection and transplantation
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: #36
tissue: liver
tissue subtype: non tumoral
|
| Extracted molecule |
total RNA |
| Extraction protocol |
liver from human biospies and transplantation
Total RNA was extracted using Mirvana extraction kit following manufacturer protocol
|
| Label |
Cy5
|
| Label protocol |
labelling protocol was agilent protocol 'G4140-90050_GeneExpression_Two_Color_v6.5-mai 2010' and the labelling was pseudo mono color (1/2 of the sample with Cy3 and 1/2 of the sample with Cy5)
|
| |
|
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanner Agilent G2565CA Feature extraction 10.7
|
| Description |
NT_78_10291_2_3
raw data file: US82400123_253949410291_S01_GE2_107_Sep09_2_3.txt
|
| Data processing |
Feature extraction 10.7 with protocol GE_107_sep09 and grid 039494_G2_D_F_20120628. Both positive and negative control probes were excluded from data normalization and data was filtered by the average of the median of the samples (79.1490322580645). The features which at least 50% of the samples have values above the cut were included (resulting in 28,782 data rows in the sample data table).
|
| |
|
| Submission date |
May 22, 2013 |
| Last update date |
May 23, 2013 |
| Contact name |
Boris HALGAND |
| E-mail(s) |
boris.halgand@univ-nantes.fr
|
| Organization name |
INSERM
|
| Street address |
1, rue Gaston Veil
|
| City |
Nantes |
| ZIP/Postal code |
44000 |
| Country |
France |
| |
|
| Platform ID |
GPL16699 |
| Series (1) |
| GSE47197 |
Human liver infected by HBV: tumoral part vs non tumoral part of the liver |
|
