|
| Status |
Public on Feb 13, 2014 |
| Title |
M_GMCSF_TPP_IFNb_IFNg_72h [5376074038_K] |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
initial differentiation: GM-CSF
activation stimuli: IFNb 100U/ml + TNFa 800 u/ml+ PGE2 1µg/ml+ P3C 1µg/ml + IFNg 200U/ml
time: 72h
donor: BC79
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Macrophage
|
| Data processing |
The data were log2-transformed and normalized using quantile normalization with Partek Genomics Suite
|
| |
|
| Submission date |
May 14, 2013 |
| Last update date |
Feb 13, 2014 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE46903 |
Transcriptome-based network analysis reveals a spectrum model of human macrophage activation [Expression] |
| GSE47189 |
Transcriptome-based network analysis reveals a spectrum model of human macrophage activation |
|
