|
| Status |
Public on Dec 01, 2013 |
| Title |
Xist KO GS_2 |
| Sample type |
RNA |
| |
|
| Source name |
GS cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Xist KO
cell type: germ line stem cells
genetic background: DBA/2
|
| Treatment protocol |
GS and ES cells were treated with trypsin and collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol (Invutrogen) and purified by RNAeasy column (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Cy3-labeled RNA probes were hybridized to a whole mouse genome DNA oligo microarray (4 × 44K, Agilent) for 16 hours at 65°C according to the manufacturer’s instructions.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner at a 5 um resolution.
|
| Description |
Gene expression of Xist KO GS cells
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software (Agilent).
|
| |
|
| Submission date |
May 02, 2013 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Jiyoung Lee |
| Organization name |
Tokyo Medical and Dental University
|
| Street address |
1-5-45 Yushima
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE46589 |
Comparison of gene expression between Xist KO GS cells and wild type GS |
|
