|
Status |
Public on Apr 30, 2016 |
Title |
NL114 |
Sample type |
RNA |
|
|
Source name |
Normal lung tissue adjacent to lung adenocarcinoma
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 58 smoking status: Non-smoking
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with QIAGEN RNeasy mini kit according to protocol provided in the kit by the manufacturer. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Data processing |
Data preprocessing of the microarray expression data included standard quality control recommended by Illumina, model based background correction (Ding et al, 2008), and surrogate variable analysis (Leek et al., 2012) to detect and alleviate batch effects33.
|
|
|
Submission date |
May 01, 2013 |
Last update date |
Apr 30, 2016 |
Contact name |
Shih Sheng Jiang |
E-mail(s) |
ssjiang@nhri.org.tw
|
Organization name |
National Health Research Institutes
|
Department |
National Institute of Cancer Research
|
Street address |
No. 35 Keyan Road, Zhunan County
|
City |
Miaoli County |
ZIP/Postal code |
350 |
Country |
Taiwan |
|
|
Platform ID |
GPL14951 |
Series (1) |
GSE46539 |
Genetic modifiers of progression-free survival in never-smoking lung adenocarcinoma patients treated with first-line TKIs |
|