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Sample GSM1124656 Query DataSets for GSM1124656
Status Public on Apr 20, 2013
Title D_133A_105_nAcc_0_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Nucleus Accumbens
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 105
brain region: nucleus accumbens
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.162816219
10001_at 5.581420803
10002_at 4.929096925
10003_at 5.301705136
100048912_at 5.639984374
10004_at 5.354291292
10005_at 7.133415133
10006_at 7.671656534
10007_at 7.346695865
10009_at 4.554676985
1000_at 7.949040401
10010_at 6.248399089
100127886_at 4.221511551
100127972_at 4.866420969
100128008_at 6.596611212
100128062_at 8.693848885
100128414_at 4.273948937
100128919_at 6.678944828
100129015_at 4.635048815
100129128_at 5.524644649

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124656_D_133A_105_nAcc_0_C_p.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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