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Sample GSM1124587 Query DataSets for GSM1124587
Status Public on Apr 20, 2013
Title M_133A_105_HC_0_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Hippocampus
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133P
subject id: 105
brain region: hippocampus
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 4.714658518
10001_at 5.106744593
10002_at 5.102930297
10003_at 4.628524278
100048912_at 6.02030334
10004_at 5.314375107
10005_at 7.02808553
10006_at 7.615632045
10007_at 7.269264732
10009_at 4.372760182
1000_at 7.73612276
10010_at 5.60681102
100127886_at 3.979848093
100127972_at 4.927940833
100128008_at 6.548337508
100128062_at 8.77226976
100128414_at 4.521863792
100128919_at 6.263590928
100129015_at 4.499958525
100129128_at 4.361104661

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124587_M_133A_105_HC_0_C_p.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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