|
| Status |
Public on Apr 20, 2013 |
| Title |
D_133A_27_HC_0_C_p |
| Sample type |
RNA |
| |
|
| Source name |
Post-mortem human brain tissue: Hippocampus
|
| Organism |
Homo sapiens |
| Characteristics |
genechip assay site: D
original genechip type: U133P
subject id: 27
brain region: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Data processing |
The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
|
| |
|
| Submission date |
Apr 17, 2013 |
| Last update date |
Apr 20, 2013 |
| Contact name |
Andy W Lin |
| E-mail(s) |
andylin@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
MBNI
|
| Lab |
Microarray
|
| Street address |
205 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48103 |
| Country |
USA |
| |
|
| Platform ID |
GPL17027 |
| Series (1) |
| GSE45642 |
Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set] |
|
