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Sample GSM1124464 Query DataSets for GSM1124464
Status Public on Apr 20, 2013
Title M_133A_48_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 48
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.149571076
10001_at 5.454534819
10002_at 5.243237052
10003_at 4.465990571
100048912_at 6.768795481
10004_at 5.088453034
10005_at 7.336240694
10006_at 6.78871391
10007_at 7.016113221
10009_at 4.232969311
1000_at 7.654051158
10010_at 4.988871104
100127886_at 4.153434243
100127972_at 5.023425975
100128008_at 7.873876597
100128062_at 8.349717356
100128414_at 4.226995509
100128919_at 6.395787155
100129015_at 4.868187167
100129128_at 4.73363314

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124464_M_133A_48_DLPFC_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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