|
| Status |
Public on Oct 14, 2014 |
| Title |
Breast Cancer Sample BC1933 |
| Sample type |
RNA |
| |
|
| Source name |
primary breast cancer tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: pretreatment excision biopsy
cell type: invasive mammary carcinoma
|
| Treatment protocol |
samples were snap frozen and stored at -80°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit, Qiagen. according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturers instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133a Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from primary breast tumors
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 16, 2013 |
| Last update date |
Oct 14, 2014 |
| Contact name |
Thomas Karn |
| E-mail(s) |
t.karn@em.uni-frankfurt.de
|
| Organization name |
J.W.Goethe-University
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4611 |
Breast Cancer Gene Expression Data from Frankfurt Series |
|
