|
| Status |
Public on Mar 24, 2014 |
| Title |
E15.5Six2GFP-S1 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic kidney
|
| Organism |
Mus musculus |
| Characteristics |
strain: hybrid of 129 and C57BL/6
tissue: kidney
cell type: nephron progenitor
genotype/variation: Six2GFP(-)
age: E15.5
|
| Treatment protocol |
Six2GFP E15.5 kidneys were disected and washed with PBS. Then FACS sorting was performed to select the GFP(+) cells and the GFP(-) cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the Trizol reagent (Life Technologies) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Fluorecent Liner Amplification kit labeling protocol. Cyanine-3 (Cy3) labeled cRNA was prepared from 0.25 ug RNA according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop Spectrophotometer.
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol. 1.65 ug of Cy3 labeled RNA was hybridized to Agilent Whole Mouse Genome microarrays (4 x 44K: GPL10333) at 60C for 17 hours and subsequently washed according to the Agilent standard hybridization protocol.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2539A Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software
|
| Description |
gene expression after tamoxifen treatment
|
| Data processing |
75 Percentile Shift Normalization by using GeneSpring GX software (Agilent Technologies)
|
| |
|
| Submission date |
Apr 01, 2013 |
| Last update date |
Mar 24, 2014 |
| Contact name |
Ryuichi Nishinakamura |
| E-mail(s) |
ryuichi@gpo.kumamoto-u.ac.jp
|
| Phone |
+81-96-373-6615
|
| Fax |
+81-96-373-6618
|
| Organization name |
Kumamoto Univ.
|
| Department |
IMEG
|
| Lab |
kidney development
|
| Street address |
Honjo 2-2-1
|
| City |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (2) |
| GSE45664 |
Six2GFPE15.5 110309 |
| GSE45845 |
Sall1 co-operated with Six2 to actively maintain nephron progenitors in the embryonic kidney |
|
