|
| Status |
Public on Mar 26, 2013 |
| Title |
OPT_31-1 month |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
treatment group: OPT
time point: 1 month
responder: NO
sex: F
age: 27
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 10 cc whole blood using the PAXgene Blood RNA Isolation kit (QIAGEN, Valencia, CA) per the manufacturer's instructions, and depleted of globin mRNA message using GLOBINclear hybridization capture technology (Ambion, Austin, TX). Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
189-7132.2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 25, 2013 |
| Last update date |
Mar 26, 2013 |
| Contact name |
Robert D Beech |
| E-mail(s) |
robert.beech@yale.edu
|
| Organization name |
Yale University
|
| Department |
Psychiatry
|
| Street address |
34 Park St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE45484 |
Gene-expression differences in peripheral blood between lithium responders and non-responders in the “Lithium Treatment -Moderate dose Use Study” (LiTMUS) |
|
