|
| Status |
Public on May 01, 2013 |
| Title |
E13.5PGCs_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
PGCs derived from mouse E13.5 embryo genital ridges
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: MCH x C57BL6/J (Oct4ΔPE-GFP Tg)
gender: male
cell type: primordial germ cells (PGCs)
|
| Treatment protocol |
The genital ridges were dissected from mouse E13.5 embryos. After trypsin treatment, live, single and GFP-positive cells were purified using FACS from the dissociated cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
|
| |
|
| Hybridization protocol |
1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
|
| Scan protocol |
The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C); the spot signals were quantified using Feature Extraction software.
|
| Description |
Gene expression of PGCs from mouse E13.5 embryo genital ridges.
|
| Data processing |
Data was normalized and analyzed by GeneSpring GX (Agilent).
|
| |
|
| Submission date |
Mar 14, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Ikuma Maeda |
| E-mail(s) |
ikuma@idac.tohoku.ac.jp
|
| Phone |
+81-22-717-8572
|
| Fax |
+81-22-717-8573
|
| Organization name |
Institute of Development, Aging and Cancer, Tohoku University
|
| Lab |
Cell Resource Center for Biomedical Research
|
| Street address |
4-1 Seiryomachi, Aoba-ku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE45181 |
Max is a repressor of germ-cell-related gene expression in mouse embryonic stem cells |
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