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Sample GSM1098618 Query DataSets for GSM1098618
Status Public on May 01, 2013
Title E13.5PGCs_rep3
Sample type RNA
Source name PGCs derived from mouse E13.5 embryo genital ridges
Organism Mus musculus
Characteristics strain/background: MCH x C57BL6/J (Oct4ΔPE-GFP Tg)
gender: male
cell type: primordial germ cells (PGCs)
Treatment protocol The genital ridges were dissected from mouse E13.5 embryos. After trypsin treatment, live, single and GFP-positive cells were purified using FACS from the dissociated cells.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C); the spot signals were quantified using Feature Extraction software.
Description Gene expression of PGCs from mouse E13.5 embryo genital ridges.
Data processing Data was normalized and analyzed by GeneSpring GX (Agilent).
Submission date Mar 14, 2013
Last update date May 01, 2013
Contact name Ikuma Maeda
Phone +81-22-717-8572
Fax +81-22-717-8573
Organization name Institute of Development, Aging and Cancer, Tohoku University
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryomachi, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (1)
GSE45181 Max is a repressor of germ-cell-related gene expression in mouse embryonic stem cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -0.26325512
DarkCorner 0.80770254
A_55_P1989846 1.9148588
A_55_P1991598 0.8509159
A_55_P2022211 -0.22811508
A_55_P1980764 -1.2674084
A_55_P1964375 0.2288208
A_51_P128876 -0.10029888
A_55_P2121042 0.7026949
A_52_P219230 0.70476675
A_51_P207591 -2.4838347
A_55_P2131920 0.7087059
A_55_P2404223 0.92206764
A_55_P2101944 -1.2367744
A_52_P358860 0.34918404
A_51_P119031 -6.39E-05
A_51_P309854 -0.40961313
A_51_P343900 0.47197056
A_51_P234359 -0.71958065
A_51_P487813 -1.2432575

Total number of rows: 39485

Table truncated, full table size 920 Kbytes.

Supplementary file Size Download File type/resource
GSM1098618_252665511681_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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