|
| Status |
Public on Jun 20, 2013 |
| Title |
N171-HD82Q (10 weeks), hippocampus, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus 10 weeks transgenic
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hippocampus
age: 10 weeks
gender: male
genotype: N171-HD82Q
genetic background: DBA and C57BL/6J
|
| Treatment protocol |
Animals were sacrificed by cervical dislocation and brains were immediately removed. Cerebella and hippocampi were dissected and placed into RNAlater solution (Qiagen) until RNA purification.
|
| Growth protocol |
The transgenic strain PrP-htt-N171-82Q (Schilling et al, 1999) (http://www.informatics.jax.org/reference/allele/MGI:2670626) was acquired from Jackson Laboratories (Sacramento, CA, USA) and maintained in a DBA and C57BL/6J mixed background (50:50) because the viability of the strain is severely compromised in a pure C57BL/6J background. Experimental protocols were consistent with European regulations and approved by the Institutional Animal Care and Use Committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted separately from individual hippocampi using TRI reagent (Sigma-Aldrich) following manufacturers' instructions. Equal amounts of total RNA from 3-4 animals were pooled and treated with RNAse-free DNaseI (Qiagen) treatment and a cleaned up using RNeasy RNA purification columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Microarray data were background corrected, normalized, summarized, and statistically analyzed using R (http://www.r-project.org/; The R Development Core Team) and Bioconductor (Gentleman et al, 2004). Mouse Gene 1.0 ST arrays were read using oligo package, using RMA algorithm as normalization method.
|
| |
|
| Submission date |
Feb 13, 2013 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Angel Barco |
| Organization name |
Instituto de Neurociencias (UMH-CSIC)
|
| Street address |
Av. Santiago Ramón y Cajal
|
| City |
Sant Joan d'Alacant |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE44306 |
Gene expression profile of N171-HD82Q hippocampus and cerebellum. |
| GSE44855 |
Genomic landscape of transcriptional and epigenetic dysregulation in a mouse model of early onset Huntington's disease |
|
